Bacterial culture media can be classified on the basis of composition, consistency, and purpose classification of bacterial culture media on the basis of consistency solid medium. Shop online for a wide selection of bd bbl mycoflask selenitef broth prepared media for isolation of salmonella from feces, urine, water, foods and other materials of sanitary. The fermentation of mannitol by salmonellae is said to correct the alkaline ph swing which can occur during incubation. Criterion selenite cystine broth for the detection of. Sodium selenite is the inorganic compound with the formula na 2 seo 3. Add aseptically 10 g of the food product after thorough mixing of the sample to 100 ml of selenite broth and add a further 10 g to 100 ml of tetrathionate broth. Selenite f broth eb02e typical formula grams per litre peptone 5. Sodium selenite bioreagent, suitable for cell culture catalog number s5261 storage at room temperature cas rn 10102188 synonym. Rv medium made from its individual ingredients and incubated at 42 degrees c was compared with a commercial preparation of sc broth. Selenite cystine broth is a modifica tion north and bartram, 1953 of the original leifson 1936 formula, differing only in the addition of lcystine which is considered to enhance salmonella growth by reduction of toxicity. Selenite ion, anion is a selenium oxoanion with the chemical formula seo 3 2. Selenite f broth composition, principle, uses, preparation and result interpretation. Composition ingredients gms litre part a tryptone 5.
It used as a source of selenium, especially for patients. New selenite selective enrichment medium for the isolation of typhoid and paratyphoid bacilli. Solid medium contains agar at a concentration of 1. It is a buffered lactose peptone broth to which sodium biselenite is added as the selective agent. Selenite broth was developed by leifson for enrichment of salmonella from sources such as feces, urine, and food. It is recommended by bis committee composition ingredients gms litre part a peptone 5. Selenite broth selenitef broth is used as an enrichment medium buffered with lactose peptone broth to which sodium biselenite is added as the selective agent for the isolation of salmonella from feces, urine, water, foods and other materials of sanitary importance. Handbook of media for clinical and public health microbiology. Selenite broth is used as an enrichment medium for the isolation of salmonella and shigella from stool, urine, water and food products. Casein enzymic hydrolysate provides nitrogenous substances and other essential ingredients. Technical information selenite broth selenite f broth twin pack product code. The pentahydrate na 2 seo 3 h 2 o 5 is the most common watersoluble selenium compound. Enzymatic digest of casein and animal tissue supply nitrogenous substances and carbohydrates for bacterial growth.
Tryptone atau polypeptone 5 g lactose 4 g sodium acid selenite nahso4 4 g na2hpo4 10 g lcystine 0,01 g aquadest 1 liter panaskan sampai mendidih agar melarut. A selenium compound with the molecular formula h2so3. Selenite f broth is recommended as an enrichment medium for the isolation of salmonella species from faeces, urine or other pathological materials in accordance with indian pharmacopoeia. Nutrient broth contains same ingredients except agar.
Beef extract is an aqueous extract of lean beef tissues. Silver selenite, an inorganic compound of formula ag 2 seo 3. This dehydrated culture medium is a raw material intended to be used in the making of prepared media products, which will require further processing, additional ingredients, or supplements. Selenite f broth is the medium used for the selective enrichment of salmonella spp from both clinical and food samples. Not all products are available for sale in all territories. Page 1 of 7 sds 7155 rev 0 june 2016 section 1 identification of the substancemixture and of the company 1. F in selenite f broth is faeces, because it is mostly used for the isolation of salmonella and shigella from faecal specimens. Subcultures should be made from the top of the broth after not more than 24 hours incubation as after this time there is. Selenite broth base manufacturer trade name selenite broth base product no. Section 1 identification of the substancemixture and of. Before antibiotic therapy, strontium selenite broth alone yieldeds. Composition of selenite f broth casein enzymic hydrolysate 5 gml lactose 4 gml sodium phosphate 10 gml sodium hydrogen selenite nahseo3 4. Selective enrichment of salmonella from foods format. Intended use hardy diagnostics criterion selenite cystine broth is a selective enrichment for isolation of salmonella spp.
Selenite f broth culture media online microbiology notes. Selenite broth is recommended as enrichment media for the isolation of salmonellae from faeces, urine or other pathological materials. Chemtrec usa 8004249300 safety data sheet r454582 supplier oxoid limited wade road basingstoke. Selenite broth selenite f broth twin pack m052 intended use. Further study of strontium selenite and selenite f broths. Remel selenite cystine broth thermo fisher scientific. Selenite f broth is widely used as an enrichment medium for the. Sodium biselenite acts as the selective agent, while lactose is added as a fermentable carbohydrate to prevent a rise in ph value during incubation because any increase in ph will reduce the selective activity of selenite. A rise in ph decreases selective activity of selenite. Selenite cystine broth is an internationally accepted medium see icmsf, 1978.
Selenite lactose broth is used for enrichment culture of salmonellae from feces, food products, water and other materials. Can we use selenite f broth media to revive organisms like. The relative effectiveness of rappaportvassiliadis rv medium, selenite cystine sc broth, and tetrathionate tt broth for the recovery of salmonella spp. Selenitef broth was devised by leifson, who demonstrated that selenite was inhibitory for coliforms and certain other microbial species, such as fecal streptococci, present in. Summary and explanation leifson found that selenite inhibited fecal streptococci and coliforms during the first 812 hours of incubation, thereby. Selenite f broth principle, uses, preparation and result. Selenite f broth twin pack m025s recommended is used as an enrichment medium for isolation of shigella from food samples. Sodium hydrogen selenite or detecting faecal coliforms drinking in water waste water, seawater and foods samples by mpn method. Of these, 96 76% were isolated with selenite f broth and 118 94% were isolated with strontium selenite broth. Remel and oxoid products are now part of the thermo scientific brand, combining powerful manual, semiautomated and fully automated test. Pancreatic digest of casein 5gl, lactose 4gl, sodium selenite 4gl, sodium phosphate 10gl. Summary and explanation selenite broth was devised by leifson,1 who demonstrated that selenite was inhibitory for coliforms and certain other microbial.
Selenite mannitol broth is an alternative to selenite f broth for the selective enrichment of salmonellae spp from clinical, food and environmental specimens. If possible even larger samples are preferable with proportionately larger amounts of the enrichment broths being used. Selenite broth selenitef broth laboratoires humeau. Reduce media preparation time and simultaneously enrich and isolate salmonella spp. Based on buffered mannitol broth, it is made selective by the addition of sodium biselenite. Selenite broth selenite f broth twin pack himedia laboratories. Composition ingredients gramslitre ingredients gramslitre part a peptic digest of animal tissue 5. It is the most widely used enrichment medium to isolate salmonella typhi from fecal specimen of suspected patients andor from carriers during salmonella outbreaks. Tm2810m mannitol selenite broth 10ml 16x125mm screw cap tube 10pkg tm2815l mannitol selenite broth 15ml 20x150mm screw cap tube 10pkg references 1. Remove the need for pipetting, dispensing or autoclaving with convenient, prefilled and readytouse tubes of selenite broth. Selenitef broth is an enrichment medium for the isolation of salmonella and some species of shigella.
Characteristics of the components used in nutrient agarbroth. Recommended as enrichment media for the isolation of salmonellae from faeces, urine or other pathological materials. Mccance, in laboratory methods in microbiology, 1966. They recommended the use of this principle for the examination of sewage and river water containing large numbers of.
Selenite broth is used as a selective medium for the isolation of salmonella species. Selenite broth is used as a selective enrichment for the cultivation of. At the same time, the number of false positive isolates accruing to strontium selenite broth. Summary and explanation selenite broth was devised by leifson,1 who demonstrated that. Can we use selenite f broth media to revive organisms like pseudomonas aeruginosa, staphylococcus aureus, micrococcus spp. Selenite broth selenite f broth is used as an enrichment medium for the isolation of salmonella from feces, urine, water, foods and other materials of sanitary importance. Withdraw approximately 1ml of the supernatant and inoculate 10 ml of selenite broth. The efficiency of strontium selenite and selenite f broths in the isolation of s. Selenite broth was originated by leifson, while observing good recovery of.
Selenite cystine broth for microbiology sigmaaldrich. Dulcitol selenite broth selenitef broth w dulcitol. Selenite f broth principle, uses, preparation and result interpretation. Selenite broth is used as a selective enrichment for the cultivation of salmonella spp. Selenite was found to selenite was found to inhibit coliforms and fecal streptococci.
Remel selenite cystine broth is selective enrichment broth for isolation of salmonella spp. A medium for the selective enrichment of salmonella spp from both clinical and food samples. Selenite broth selenitef broth is used as an enrichment medium for the isolation of salmonella from feces, urine, water, foods and other materials of sanitary importance. Pi7283, rev 05, march 2008 selenite cystine broth 7283 intended use selenite cystine broth is used for the selective enrichment of salmonella species. Selenous acid, the conjugate acid, with the formula h 2 seo 3.
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